Last modified by rbakker on 2022/05/23 22:23
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... ... @@ -3,7 +3,7 @@ 3 3 (% class="container" %) 4 4 ((( 5 5 = Use case: 6 - Reconstructed long-range projection neuron and 33 section images =6 +Long-range projection neuron and 33 section images = 7 7 8 8 Pipeline performing coregistration of the neuron and the 2d-sections from which it was traced 9 9 ))) ... ... @@ -15,13 +15,27 @@ 15 15 ((( 16 16 = What can I find here? = 17 17 18 -* Notice how the table of contents on the right 19 -* is automatically updated 20 -* to hold this page's headers 18 +This pipeline is dedicated to coregister 3D neuronal morphology traced in Neurolucida into the Allen atlas, making use of 2d-sections from which it was traced. 21 21 20 +The pipeline consists of several steps: 21 + 22 +- neuron and image preprocessing; 23 + 24 +- projection of neuron-points into pixel coordinates in the associated experimental slice; 25 + 26 +- QuickNII mapping between the atlas and the experimental slice; 27 + 28 +- Elastix coregistration of images and, consequently, neuron-points; 29 + 30 +- linear mapping of the reconstructed neuron into the original shape; 31 + 32 +- evaluation of the reconstructed neuron by comparison with the same neuron coregistered with a corresponding point technique, and with the ground truth extracted by the neuroanatomist. 33 + 34 +The code, intermediate and final results are stored in this collection. 35 + 22 22 = For whom is this interesting? = 23 23 24 - Describeheaudiencefthiscollab.38 +Neuroscientists and neuroanatomists interested in ... 25 25 ))) 26 26 27 27