Last modified by rbakker on 2022/05/23 22:23
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... ... @@ -3,9 +3,9 @@ 3 3 (% class="container" %) 4 4 ((( 5 5 = Use case: 6 - Long-range projection neuron and 33 section images =6 +Reconstructed long-range projection neuron and 33 section images = 7 7 8 -Pipeline performing coregistration of the neuron and the 2d-sections from which it was traced 8 +Pipelinne performing coregistration of the neuron and the 2d-sections from which it was traced 9 9 ))) 10 10 ))) 11 11 ... ... @@ -15,27 +15,13 @@ 15 15 ((( 16 16 = What can I find here? = 17 17 18 -This pipeline is dedicated to coregister 3D neuronal morphology traced in Neurolucida into the Allen atlas, making use of 2d-sections from which it was traced. 18 +* Notice how the table of contents on the right 19 +* is automatically updated 20 +* to hold this page's headers 19 19 20 -The pipeline consists of several steps: 21 - 22 -- neuron and image preprocessing; 23 - 24 -- projection of neuron-points into pixel coordinates in the associated experimental slice; 25 - 26 -- QuickNII ([[https:~~/~~/ebrains.eu/service/quicknii-and-visualign/>>https://ebrains.eu/service/quicknii-and-visualign/]]) mapping between the atlas and the experimental slice; 27 - 28 -- Elastix ([[https:~~/~~/simpleelastix.github.io/>>https://simpleelastix.github.io/]]) coregistration of images and, consequently, neuron-points; 29 - 30 -- linear mapping of the reconstructed neuron into the original shape; 31 - 32 -- evaluation of the reconstructed neuron by comparison with the same neuron coregistered with a corresponding point technique, and with the ground truth extracted by the neuroanatomist. 33 - 34 -The code, intermediate and final results are stored in this collection. 35 - 36 36 = For whom is this interesting? = 37 37 38 - Neuroscientistsand neuroanatomists working withneuronalmorphologiesand who wouldliketo register their data to anatlas. In particular theAllen mouse atlas is very interesting because large numbers ofneurons are already available inthat space from two largeinitiatives:Mouselight ([[https:~~/~~/www.janelia.org/project-team/mouselight>>https://www.janelia.org/project-team/mouselight]]) and Braintell ([[https:~~/~~/doi.org/10.35077/g.25>>https://doi.org/10.35077/g.25]]).24 +Describe the audience of this collab. 39 39 ))) 40 40 41 41
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