Last modified by rbakker on 2022/05/23 22:23
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... ... @@ -2,9 +2,10 @@ 2 2 ((( 3 3 (% class="container" %) 4 4 ((( 5 -= Coregistration of a neuron and the 2d-sections from which it was traced = 5 += Use case: 6 +Long-range projection neuron and 33 section images = 6 6 7 - Coregistration ofaneuron and the 2d-sections from which it was traced8 +Pipeline performing coregistration of the neuron and the 2d-sections from which it was traced 8 8 ))) 9 9 ))) 10 10 ... ... @@ -14,13 +14,27 @@ 14 14 ((( 15 15 = What can I find here? = 16 16 17 -* Notice how the table of contents on the right 18 -* is automatically updated 19 -* to hold this page's headers 18 +This pipeline is dedicated to coregister 3D neuronal morphology traced in Neurolucida into the Allen atlas, making use of 2d-sections from which it was traced. 20 20 20 +The pipeline consists of several steps: 21 + 22 +- neuron and image preprocessing; 23 + 24 +- projection of neuron-points into pixel coordinates in the associated experimental slice; 25 + 26 +- QuickNII mapping between the atlas and the experimental slice; 27 + 28 +- Elastix coregistration of images and, consequently, neuron-points; 29 + 30 +- linear mapping of the reconstructed neuron into the original shape; 31 + 32 +- evaluation of the reconstructed neuron by comparison with the same neuron coregistered with a corresponding point technique, and with the ground truth extracted by the neuroanatomist. 33 + 34 +The code, intermediate and final results are stored in this collection. 35 + 21 21 = For whom is this interesting? = 22 22 23 - Describeheaudiencefthiscollab.38 +Neuroscientists and neuroanatomists interested in ... 24 24 ))) 25 25 26 26