Wiki source code of Atlas registration of neuronal morphologies obtained from mouse brain sections
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10.1 | 5 | = Use case: |
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13.1 | 6 | Long-range projection neuron and 33 section images = |
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12.1 | 8 | Pipeline performing coregistration of the neuron and the 2d-sections from which it was traced |
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16 | = What can I find here? = | ||
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14.1 | 18 | This pipeline is dedicated to coregister 3D neuronal morphology traced in Neurolucida into the Allen atlas, making use of 2d-sections from which it was traced. |
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14.1 | 20 | The pipeline consists of several steps: |
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22 | - neuron and image preprocessing; | ||
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24 | - projection of neuron-points into pixel coordinates in the associated experimental slice; | ||
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15.1 | 26 | - QuickNII ([[https:~~/~~/ebrains.eu/service/quicknii-and-visualign/>>https://ebrains.eu/service/quicknii-and-visualign/]]) mapping between the atlas and the experimental slice; |
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15.1 | 28 | - Elastix ([[https:~~/~~/simpleelastix.github.io/>>https://simpleelastix.github.io/]]) coregistration of images and, consequently, neuron-points; |
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30 | - linear mapping of the reconstructed neuron into the original shape; | ||
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32 | - evaluation of the reconstructed neuron by comparison with the same neuron coregistered with a corresponding point technique, and with the ground truth extracted by the neuroanatomist. | ||
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34 | The code, intermediate and final results are stored in this collection. | ||
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8.2 | 36 | = For whom is this interesting? = |
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14.1 | 38 | Neuroscientists and neuroanatomists interested in ... |
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44 | {{box title="**Contents**"}} | ||
45 | {{toc/}} | ||
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