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5	5	Ilastik is a versatile image analysis tool specifically designed for the classification, segmentation and analysis of biological images based on supervised machine learning algorithms.
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	7	+**There are two main approaches for the analysis of rodent brain section image**
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18		-There are two main approaches for the analysis of rodent brain section image
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20	20	1. Pixel classification only (with two or more classes)
21		-1. Pixel classification with two classes (//immunoreactivity// and //background//), followed by object classification with two classes (//objects of interest// and //artefact//).
	10	+1. Pixel classification with two classes (//immunoreactivity// and //background//), followed by Object classification with two classes (//objects of interest// and //artefact//).
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23	23	**Which approach is best for my dataset?**
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35	35	* Export the probability maps in HDF5 format, and simple_segmentation images in PNG format with the default settings.
36	36	* Review the results.
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56	56	=== (% style="color:#c0392b" %)Object classification workflow(%%) ===
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58	58	There are three options on the ilastik start up page for running Object Classification.  Choose the //Object Classification with Raw Data and Pixel Prediction Maps as input//**.**