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Version 2.2 by puchades on 2020/03/25 14:54
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1 == Analysis approach for series of rodent brain section image ==
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3 There are two main approaches for the analysis of rodent brain section images.
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5 1. Pixel classification only (with two or more classes)
6 1. Pixel classification with two classes (//immunoreactivity// and //background//), followed by object classification with two classes (//objects-of-interest// and //artefact//).
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8 **Which approach is best for my dataset?**
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10 As a general rule, pixel classification is suitable for images in which there are clear differences in the colour, intensity and/ or texture of the feature-of-interest versus the background and other structures.  If there is non-specific labelling in the image that is very similar in appearance to the labelling-of-interest, object classification may allow the non-specific labelling to be filtered out based on object level features such as size and shape. The best approach is determined by trial and error.
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12 === Pixel classification workflow ===
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14 For a quick introduction, watch: [[https:~~/~~/www.youtube.com/watch?v=5N0XYW9gRZY&feature=youtu.be>>url:https://www.youtube.com/watch?v=5N0XYW9gRZY&feature=youtu.be]]
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16 === Object classification workflow ===
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19 ==== ====
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