Changes for page 5. Quantification and spatial analysis with Nutil
Last modified by puchades on 2021/11/16 09:11
Summary
-
Page properties (2 modified, 0 added, 0 removed)
-
Attachments (0 modified, 2 added, 1 removed)
Details
- Page properties
-
- Author
-
... ... @@ -1,1 +1,1 @@ 1 -XWiki. sharoncy1 +XWiki.puchades - Content
-
... ... @@ -2,8 +2,35 @@ 2 2 3 3 A detailed user manual is included when downloading the software. 4 4 5 -[[image: Quantifier.PNG]]5 +[[image:Nutil_GUI.png||style="float:left"]] 6 6 7 + 8 + 9 + 10 + 11 + 12 + 13 + 14 + 15 + 16 + 17 + 18 + 19 + 20 + 21 + 22 + 23 + 24 + 25 + 26 + 27 + 28 + 29 + 30 + 31 + 32 + 33 + 7 7 **Basic steps:** 8 8 9 9 **//Nutil Quantifier//** quantifies and assigns anatomical location to labelled objects identified by segmentation in rodent brain section images (mouse or rat). ... ... @@ -10,12 +10,12 @@ 10 10 11 11 It requires the following input: 12 12 13 -1. (% style="color:#c0392b" %)**Segmentations with the labelling displayed in one RGB colour**40 +1. (% style="color:#c0392b" %)**Segmentations with the labelling displayed in a single RGB colour** 14 14 15 15 **Requirement:** 16 16 17 -* mustbe24-bit RGB images in PNGformat.18 -* same pr oportionsas the images used in QuickNII to generate the atlas maps (can be a different size).44 +* Input format: 24-bit RGB images in PNG 45 +* The images have to have the same aspect ratio as the images used in QuickNII to generate the atlas maps (can be a different size). 19 19 * //Quantifier** **//is only able to extract one RGB colour at a time. Apply one RBG colour to all of the objects of interest, and specify this colour co//d//e in the GUI (e.g. 255, 0, 0 for red). 20 20 21 21 To generate the segmentations we recommend the //Pixel and// //Object Classification workflows// in the //ilastik// software (//ilastik.org//). However, any image analysis software may be used. ... ... @@ -28,12 +28,12 @@ 28 28 (% start="3" %) 29 29 1. (% style="color:#c0392b" %)**Anchored XML**(%%) file generated with the //QuickNII// software. 30 30 31 -[[image:Illustration seg_flat_files.png ||height="254" width="500"]]58 +[[image:Illustration seg_flat_files.png]] 32 32 60 +== (% style="color:#c0392b" %)Reference atlases in //Nutil Quantifier//(%%) == 33 33 34 -== (% style="color:#c0392b" %)Reference atlases available in //Nutil Quantifier//(%%) == 35 35 63 +The Nutil software is currently compatible with: 36 36 37 -The Nutil software is currently compatible with the Allen mouse brain Atlas v3 (2015 or 2017) and the Waxholm Space Atlas of the Sprague Dawley Rat v2 or v3. 38 - 39 - 65 +* Allen mouse brain Atlas (CCFv3, 2015 or 2017) 66 +* Waxholm Space Atlas of the Sprague Dawley rat brain v2 or v3.
- Quantifier.PNG
-
- Author
-
... ... @@ -1,1 +1,0 @@ 1 -XWiki.puchades - Size
-
... ... @@ -1,1 +1,0 @@ 1 -37.8 KB - Content
- Nutil_GUI.png
-
- Author
-
... ... @@ -1,0 +1,1 @@ 1 +XWiki.puchades - Size
-
... ... @@ -1,0 +1,1 @@ 1 +49.2 KB - Content
- Nutil_main.PNG
-
- Author
-
... ... @@ -1,0 +1,1 @@ 1 +XWiki.puchades - Size
-
... ... @@ -1,0 +1,1 @@ 1 +51.2 KB - Content