Last modified by puchades on 2021/11/16 09:11

From version 5.4
edited by puchades
on 2020/03/25 17:06
Change comment: There is no comment for this version
To version 2.4
edited by puchades
on 2020/03/25 15:30
Change comment: There is no comment for this version

Summary

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3 3  A detailed Nutil user manual is included when downloading the software.
4 4  
5 -[[image:Quantifier.PNG]]
6 -
7 7  **Basic steps:**
8 8  
9 -**//Quantifier//** locates and quantifies labeling identified by segmentation in rodent brain section images (mouse or rat). It requires the following input:
7 +**//Quantifier//** locates and quantifies labelling identified by segmentation in rodent brain section images (mouse or rat). It requires the following input:
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11 11  (% start="1" style="list-style-type:decimal" %)
12 -1. **Segmentations with the labeling displayed in one RGB colour**
10 +1. **Segmentations with the labelling displayed in one RGB colour **(Left panel)
13 13  
14 14  **Requirement:**
15 15  
16 16  * must be 24-bit RGB images in PNG format
17 17  * same proportions as the images used in QuickNII to generate the atlas maps (can be a different size)
18 -* //Quantifier** **//is only able to extract one RGB colour at a time. Apply one RBG colour to all of the objects of interest, and specify this colour code in the //Excel template//.
16 +* **//Quantifier //**is only able to extract one RGB colour at a time. Apply one RBG colour to all of the objects of interest, and specify this colour code in the //Excel template//.
19 19  
20 20  To generate the segmentations we recommend the //Pixel and// //Object Classification workflows// in the //ilastik// software (//ilastik.org//). However, any image analysis software may be used.
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23 -1. **Customised anatomical atlas maps**
20 +1. **Customised anatomical atlas maps **(Right panel)
24 24  
25 -Generate the atlas maps in FLAT format with the //QuickNII //software
26 26  
27 -(% start="3" %)
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24 +Generate the atlas maps in FLAT format with the //QuickNII //software ([[www.nitrc.org/projects/quicknii>>url:https://www.nitrc.org/projects/quicknii]])
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26 +
28 28  1. **Anchored XML** file generated with the //QuickNII// software.
29 -1. **Reference atlas label file** (in LABEL format) corresponding to the relevant atlas. The following reference atlases are included in the //Nutil// package (in the “Labels” folder). 
30 30  
31 -[[image:Illustration seg_flat_files.png||height="254" width="500"]]
32 32  
30 +1. **Reference atlas label file** (in LABEL format) corresponding to the relevant atlas. The following reference atlases are included in the //Nutil// package (in the “Labels” folder).
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37 +(% class="wikigeneratedid" id="HH4Won27tAppearinToC" %)
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Illustration seg_flat_files.png
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