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7	7	**Basic steps:**
8	8
9		-**//Quantifier//** locates and quantifies labeling identified by segmentation in rodent brain section images (mouse or rat).
	9	+**//Nutil Quantifier//** locates and quantifies labeling identified by segmentation in rodent brain section images (mouse or rat).
10	10
11	11	It requires the following input:
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14	14
15	15	**Requirement:**
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17		-* must be 24-bit RGB images in PNG format
18		-* same proportions as the images used in QuickNII to generate the atlas maps (can be a different size)
	17	+* must be 24-bit RGB images in PNG format.
	18	+* same proportions as the images used in QuickNII to generate the atlas maps (can be a different size).
19	19	* //Quantifier** **//is only able to extract one RGB colour at a time. Apply one RBG colour to all of the objects of interest, and specify this colour code in the //Excel template//.
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21	21	To generate the segmentations we recommend the //Pixel and// //Object Classification workflows// in the //ilastik// software (//ilastik.org//). However, any image analysis software may be used.
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23	23	(% start="2" %)
24	24	1. (% style="color:#c0392b" %)**Customised anatomical atlas maps**
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26		-Generate the atlas maps in FLAT format with the //QuickNII //software
	26	+Generate the atlas maps in FLAT format with the //QuickNII //software.
27	27
28	28	(% start="3" %)
29	29	1. (% style="color:#c0392b" %)**Anchored XML**(%%) file generated with the //QuickNII// software.