Changes for page QUINT: Workflow for Quantification and Spatial Analysis of Features in Histological Images From Rodent Brain
Last modified by puchades on 2022/11/02 10:16
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... ... @@ -1,16 +1,7 @@ 1 -(% class="jumbotron" %) 2 -((( 3 -(% class="container" %) 4 -((( 5 -== (% style="color:#16a085" %)**QUINT: Workflow for Quantification and Spatial Analysis of Features in Histological Images from Rodent Brain**(%%) == 1 +(% style="text-align:center" %) 2 +[[image:QUINT_workflow_pic.png]] 6 6 7 -(% style="color:#999999" %)Neural Systems Laboratory, University of Oslo 8 -))) 9 -))) 10 10 11 -==== ==== 12 - 13 - 14 14 ==== (% style="color:#c0392b" %)**Workflow Description**(%%) ==== 15 15 16 16 The QUINT workflow enables the quantification and spatial analysis of labelled features in histological images of rodent brain sections based on reference atlases of the brain. It utilises three open-source software: ... ... @@ -17,7 +17,7 @@ 17 17 18 18 1. [[(% style="color:#2980b9" %)//ilastik//>>doc:.3\. Image segmentation with ilastik.WebHome]](%%) allows the extraction of labelled features such as cells by segmentation. 19 19 1. [[(% style="color:#2980b9" %)//QuickNII//>>doc:.Image registration to reference atlas using QuickNII.WebHome]](%%) generates atlas maps customised to match the proportions and cutting plane of the brain sections. 20 -1. (% style="color:#2980b9" %) //Nutil//(%%) enables image [[transformations>>doc:.1\. Preparing the images.WebHome]], in addition to [[quantification and spatial analysis>>doc:.4\. Quantification and spatial analysis with Nutil.WebHome]] of features by drawing on the output of ilastik and QuickNII.11 +1. (% style="color:#2980b9" %)Nutil(%%) enables image [[transformations>>doc:.1\. Preparing the images.WebHome]], in addition to [[quantification and spatial analysis>>doc:.4\. Quantification and spatial analysis with Nutil.WebHome]] of features by drawing on the output of ilastik and QuickNII. 21 21 22 22 In combination, the tools facilitate semi-automated quantification, eliminating the need for more time consuming methods such as stereological analysis with manual delineation of brain regions. 23 23 ... ... @@ -41,4 +41,3 @@ 41 41 [[https:~~/~~/www.ilastik.org>>url:https://www.ilastik.org/]][[/>>url:https://www.ilastik.org/]] 42 42 ))) 43 43 44 -
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