Changes for page QUINT: Workflow for Quantification and Spatial Analysis of Features in Histological Images From Rodent Brain
Last modified by puchades on 2022/11/02 10:16
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... ... @@ -1,7 +1,16 @@ 1 -(% style="text-align:center" %) 2 -[[image:QUINT_workflow_pic.png]] 1 +(% class="jumbotron" %) 2 +((( 3 +(% class="container" %) 4 +((( 5 +== (% style="color:#16a085" %)**QUINT: Workflow for Quantification and Spatial Analysis of Features in Histological Images from Rodent Brain**(%%) == 3 3 7 +(% style="color:#999999" %)Neural Systems Laboratory, University of Oslo 8 +))) 9 +))) 4 4 11 +==== ==== 12 + 13 + 5 5 ==== (% style="color:#c0392b" %)**Workflow Description**(%%) ==== 6 6 7 7 The QUINT workflow enables the quantification and spatial analysis of labelled features in histological images of rodent brain sections based on reference atlases of the brain. It utilises three open-source software: ... ... @@ -8,7 +8,7 @@ 8 8 9 9 1. [[(% style="color:#2980b9" %)//ilastik//>>doc:.3\. Image segmentation with ilastik.WebHome]](%%) allows the extraction of labelled features such as cells by segmentation. 10 10 1. [[(% style="color:#2980b9" %)//QuickNII//>>doc:.Image registration to reference atlas using QuickNII.WebHome]](%%) generates atlas maps customised to match the proportions and cutting plane of the brain sections. 11 -1. (% style="color:#2980b9" %)Nutil(%%) enables image [[transformations>>doc:.1\. Preparing the images.WebHome]], in addition to [[quantification and spatial analysis>>doc:.4\. Quantification and spatial analysis with Nutil.WebHome]] of features by drawing on the output of ilastik and QuickNII. 20 +1. (% style="color:#2980b9" %)//Nutil//(%%) enables image [[transformations>>doc:.1\. Preparing the images.WebHome]], in addition to [[quantification and spatial analysis>>doc:.4\. Quantification and spatial analysis with Nutil.WebHome]] of features by drawing on the output of ilastik and QuickNII. 12 12 13 13 In combination, the tools facilitate semi-automated quantification, eliminating the need for more time consuming methods such as stereological analysis with manual delineation of brain regions. 14 14 ... ... @@ -32,3 +32,4 @@ 32 32 [[https:~~/~~/www.ilastik.org>>url:https://www.ilastik.org/]][[/>>url:https://www.ilastik.org/]] 33 33 ))) 34 34 44 +
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