Changes for page QUINT: Workflow for Quantification and Spatial Analysis of Features in Histological Images From Rodent Brain
Last modified by puchades on 2022/11/02 10:16
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... ... @@ -1,34 +3,44 @@ 1 - 2 - 3 3 (% class="jumbotron" %) 4 4 ((( 5 5 (% class="container" %) 6 6 ((( 7 -== QUINT: Workflow for Quantification and Spatial Analysis of Features in Histological Images from Rodent Brain == 5 +== (% style="color:#16a085" %)**QUINT: Workflow for Quantification and Spatial Analysis of Features in Histological Images from Rodent Brain**(%%) == 6 + 7 +(% style="color:#999999" %)Neural Systems Laboratory, University of Oslo 8 8 ))) 9 9 ))) 10 10 11 -(% class="row" %) 12 -((( 13 -(% class="col-xs-12 col-sm-8" %) 14 -((( 15 -(% class="wikigeneratedid" %) 16 -= = 11 +==== ==== 17 17 18 -= What can I find here? = 19 19 20 -* Notice how the table of contents on the right 21 -* is automatically updated 22 -* to hold this page's headers 14 +==== (% style="color:#c0392b" %)**Workflow Description**(%%) ==== 23 23 24 - =Who has access?=16 +The QUINT workflow enables the quantification and spatial analysis of labelled features in histological images of rodent brain sections based on reference atlases of the brain. It utilises three open-source software: 25 25 26 -Describe the audience of this collab. 27 -))) 18 +1. (% style="color:#2980b9" %)//ilastik//(%%) allows the extraction of labelled features such as cells by segmentation. 19 +1. (% style="color:#2980b9" %)//QuickNII//(%%) generates atlas maps customised to match the proportions and cutting plane of the brain sections. 20 +1. (% style="color:#2980b9" %)//Nutil//(%%) enables image transformations, in addition to quantification and spatial analysis of features by drawing on the output of ilastik and QuickNII. 28 28 22 +In combination, the tools facilitate semi-automated quantification, eliminating the need for more time consuming methods such as stereological analysis with manual delineation of brain regions. 29 29 30 -(% class="col-xs-12 col-sm-4" %) 24 + 25 +==== (% style="color:#c0392b" %)**Workflow highlights**(%%) ==== 26 + 27 +* The QUINT workflow is semi-automated, combining three open-source software that can be operated without scripting knowledge. 28 + 31 31 ((( 32 - 30 +As the quantifications are performed in regions defined by a reference atlas, the region definitions are standardized, allowing comparisons of data from different laboratories. 31 + 32 + 33 +==== (% style="color:#c0392b" %)**References**(%%) ==== 34 + 35 +Yates SC et al. 2019. QUINT: Workflow for Quantification and Spatial Analysis of Features in Histological Images From Rodent Brain. Front. Neuroinform. 13:75. doi: 10.3389/fninf.2019.00075 36 + 37 +[[https>>url:https://www.nitrc.org/projects/nutil/]][[:~~/~~/www.nitrc.org/projects/nutil>>url:https://www.nitrc.org/projects/nutil/]][[/>>url:https://www.nitrc.org/projects/nutil/]] 38 + 39 +[[https:~~/~~/>>url:https://www.nitrc.org/projects/quicknii]][[www.nitrc.org/projects/quicknii>>url:https://www.nitrc.org/projects/quicknii]] 40 + 41 +[[https:~~/~~/www.ilastik.org>>url:https://www.ilastik.org/]][[/>>url:https://www.ilastik.org/]] 33 33 ))) 34 -))) 43 + 44 +