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Last modified by puchades on 2022/11/02 10:16
From version 60.1
edited by evanhancock
on 2020/12/03 12:18
Change comment: There is no comment for this version
To version 66.1
edited by sharoncy
on 2021/10/18 09:45
Change comment: There is no comment for this version

Summary

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1 -[[image:QUINT_workflow_Plaques.png||style="float:left"]]
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3 +Online documentation: -
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5 +[[QuickNII user documentation>>https://quicknii.readthedocs.io/en/latest/index.html]]
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7 +[[VisuAlign user documentation>>https://visualign.readthedocs.io/en/latest/index.html]]
8 +
9 +[[Ilastik user documentation>>https://nutil.readthedocs.io/en/latest/Ilastik.html]]
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11 +[[Nutil user documentation>>https://nutil.readthedocs.io/en/latest/index.html]]                                                                   
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15 +[[image:QUINT_workflow_Plaques.png||height="470" style="float:left" width="1277"]]
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3 3  == (% style="color:#c0392b" %)**Description**(%%) ==
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5 -**The QUINT workflow enables an atlas based analysis of extracted features from histological image sections from the rodent brain using 3D reference atlases. **
37 +**The QUINT workflow enables an atlas-based analysis of extracted features from histological image sections from the rodent brain by using 3D reference atlases. **
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7 -**Examples of use are: cell counting and spatial distributions; determination of projection areas in connectivity experiments; exploration of pathological hallmarks in brain disease models. Integration of various data to the same reference space enables new exploration strategies and re-use of experimental data.**
39 +**Examples of use are cell counting and spatial distributions, determination of projection areas in connectivity experiments, and exploration of pathological hallmarks in brain-disease models. Integration of various data to the same reference space enables new exploration strategies and reuse of experimental data.**
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9 -The workflow is built on the following open access software:
41 +The workflow is built on the following open-access software.
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11 -* [[(% style="color:#2980b9" %)//ilastik//>>doc:.3\. Image segmentation with ilastik.WebHome]](%%) allows the extraction of labelled features such as cells, using machine learning image segmentation.
43 +* [[(% style="color:#2980b9" %)//ilastik//>>doc:.3\. Image segmentation with ilastik.WebHome]](%%) allows the extraction of labelled features such as cells, by using machine-learning image segmentation.
12 12  * [[(% style="color:#2980b9" %)//QuickNII//>>doc:.Image registration to reference atlas using QuickNII.WebHome]](%%) generates custom-angle slices from volumetric brain atlases to match the proportions and cutting plane of histological sections.
13 -* //[[(% style="color:#3498db" %)VisuAlign>>doc:.Image registration to reference atlas using QuickNII.WebHome]]//(%%) is then used for nonlinear alignment of the reference atlas slice to the section image..
45 +* //[[(% style="color:#3498db" %)VisuAlign>>doc:.Image registration to reference atlas using QuickNII.WebHome]]//(%%) is then used for non-linear alignment of the reference-atlas slice to the section image.
14 14  * (% style="color:#2980b9" %)//Nutil//(%%) enables image [[transformations>>doc:.1\. Preparing the images.WebHome]], in addition to [[quantification and spatial analysis>>doc:.4\. Quantification and spatial analysis with Nutil.WebHome]] of features by drawing on the output of //ilastik// and //QuickNII//.
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16 -In combination, the tools facilitate semi-automated quantification, eliminating the need for more time consuming methods such as stereological analysis with manual delineation of brain regions.
48 +In combination, the tools facilitate semi-automated quantification, eliminating the need for more time-consuming methods such as stereological analysis with manual delineation of brain regions.
17 17  
18 18  [[[[image:Youtube_QUINT.PNG||height="282" style="float:left" width="500"]]>>https://www.youtube.com/watch?v=8oeg3qTzLnE]]
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40 -The semi-automated QUINT workflow uses open access software that can be operated without any scripting knowledge.
72 +The semi-automated QUINT workflow uses open-access software that can be operated without any scripting knowledge.
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46 -As the quantifications are performed in regions defined by a reference atlas, the region definitions are standardized, allowing comparisons of data from different laboratories.
78 +Because the quantifications are performed in regions defined by a reference atlas, the region definitions are standardised, allowing comparisons of data from different laboratories.
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48 48  
49 49  ==== (% style="color:#c0392b" %)**References**(%%) ====
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